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Effect of Agnihotra on Aerial Microflora

Our way of life has intensified the quantum of pollution. No place can be called safe from pollution. What varies is the type of pollutant and the degree of pollution. Pollution is of various types such as gaseous pollution, water pollution, food pollution, radioactive pollution and so on. Of these types microbial pollution is the most important type of pollution for people in the medical or paramedical field.

Microorganisms are ubiquitous in nature. There are mainly two types, namely non-pathogenic or saprophytic (harmless and not causing any disease) and pathogenic (disease producing). There are certain opportunistic pathogens which, given a chance, can produce disease in human beings. Thus the mere presence of these microorganisms in a definite strength in various media can produce contaminants.

Microorganisms like Salmonellae, Shigellae or Vibrios contaminate water, eatables, milk and milk products. When the contaminated eatables are consumed the individual suffers from typhoid, bacillary dysentery or cholera. Similarly, organisms like Staphylococci cause food poisoning by increasing toxins in food. This microorganism also causes wound infections with pus formation. Streptococci infect the respiratory tract after inhalation of the droplet nuclei on which they are settled. Hospital infections by Staphylococci and Pseudomones are not uncommon. Recently, Pseudomonas aeruginosa has been reported to have entered the space age. This microorganism was isolated from the lining of the fuel tank of a jet engine and was found to be responsible for the corrosion of the tank.

It is stated that Agnihotra helps to undo the effects of pollution. In this respect it was decided to observe the last type of pollutant, i.e., microorganisms and the effect of Agnihotra on them. The present article restricts only to the effect of Agnihotra on microorganisms as observed in a microbiology laboratory.

Details of experiments undertaken by Dr Arvind G. Mondkar of Grant Medical College, Bombay in 1982. The attempt is to show the results of Agnihotra, a fumigation process based on the biorhythm of nature corresponding to sunrise/sunset. A copper pyramid of fixed shape and size is used in the process of Agnihotra.

Details of the Room

  • Outline shows the walls of the room.
  • A, B, C, D, E and F indicate the position of the petri plates.
  • A and D plates were on the floor, forming XY plane.
  • E and F plates were placed at 6' 9/1 above the floor of the room forming Z axis.
  • The North-South direction of the room is shown near A.
  • The distance between plates is indicated by arrows.

Bacteriological Results

  • The full names of the Microorganisms listed in the above table are given below.
  • The observations of Plate E are not in line with other five plates, so Plate E will be studied more carefully in our next experiment.
  • The number following each Microorganism indicates the number of Microorganisms of that type observed in that plate.



  • In Plate C, "D. pneumoniae" disappeared within 30 minutes.
  • In Plate A, fungal colony disappeared within 30 minutes.
  • In Plates A and D, "B. subtilis" disappeared within 60 minutes while the same organism disappeared within 30 minutes in B.
  • Rest of the organisms were reduced in their numbers.
  • There is a definite reduction in the Aerial Microflora after performing Agnihotra.

(Agnihotra atmosphere discourages the production and growth of some organisms. Such organisms try to escape from this atmosphere.)

Full Names of the Microorganisms

The complete name of the microorganism is given first and the short form is given in brackets.

  1. Staphylococcus albus (Staph. albus or St. alb.):
    Usually found on the skin. Non-pathogenic, but if they enter the body through a cut then they become pathogenic.
  2. Bacillus subtilis (B. sub):
    Non-pathogenic and found everywhere (i.e. ubiquitous).
  3. Fungus:
    Non-pathogenic, ubiquitous.
  4. Escherichia Coli (E. coli):
    Non-pathogenic, ubiquitous. It helps in Vitamin B12 synthesis in human intestine.
  5. Citrabacter freundii (Cit. fre.):
    Non-pathogenic, ubiquitous.
  6. Staphylococcus pyogenes (Staph. Pyo.):
    This is found everywhere. It is the "pus" forming organism.
  7. Diplococcus Pneumoniae (D. pneu):
    This causes Pneumonia.